CO41 | Failure in assisted reproductive techniques: insights from the first registry

E. Grandone^1,3,4, C. Lodigiani², D. Colaizzo³, M. Passaretti², J. Khizroeva⁴, A. De Laurenzo³, P. Totaro⁵, E. Bucherini⁶, D. Baldini⁷, V. Bitsadze⁴, A. Makatsarya⁴, M. Mastroianno³, G. Piazza⁸, M. Othman⁹, M. Margaglione¹ | ¹University of Foggia, Italy; ²Humanitas University, Rozzano, Italy; ³IRCCS Casa Sollievo della Sofferenza, San Giovanni Rotondo, Italy; ⁴First Sechenov University, Russia; ⁵Santa Maria Hospital, Bari, Italy; ⁶Angiologia, Faenza, Italy; ⁷Momo Fertilife, Bisceglie, Italy; ⁸Brigham Women Hospital, USA; ⁹Queen’s University, Kingston, Canada

Infertility affects approximately 9% of women aged 20-44 years, globally, with clinical pregnancy rates after embryo transfer (ET) through Assisted Reproductive Techniques (ART) ranging from 20% to 35%. Multiple factors influence pregnancy success, but evidence remains inconsistent. Prophylactic low-molecular-weight heparin (LMWH) has been explored in small randomized controlled trials for its potential to enhance outcomes and reduce thrombotic risk. Similarly, aspirin has been proposed to improve live birth rates by enhancing endometrial preparation, but findings have been inconclusive due to limited sample sizes and variability among studies. The FIRST registry aimed to assess factors associated with reproductive outcomes in women undergoing ART cycles after experiencing ≥2 implantation failures (IF) or pregnancy losses (PL). Between 2015 and 2023, 904 women were prospectively enrolled, tracking pregnancy rates, live-birth rates, and thrombotic events. Of these, 459 (50.8%) achieved a positive pregnancy test, with 422 (91.9%) resulting in intrauterine pregnancies. However, 73 (15.9%) ended in miscarriage or ectopic pregnancy. Analysis of antithrombotic treatment showed the following live-birth rates: 228/409 (55.7%) for LMWH, 92/139 (66.2%) for aspirin (ASA), and 76/102 (74.5%) for combined therapy, all with significant univariate associations compared with no treatment (p <0.001). Logistic regression adjusting for confounders (age, BMI, previous conception history, previous VTE, and antithrombotic treatment) demonstrated that live birth was negatively associated with age and positively with use of ASA, LMWH, or both (Table 1). In conclusion, among women with repeated IF or PL following ART, antithrombotic prophylaxis significantly improves the odds of live birth in the subsequent ART attempt.