Original Articles
Vol. 5 No. 2 (2026)
Neurovascular protective effects of nutritional interventions in ischemic brain injury
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Published: 10 April 2026
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Background: Cerebral ischemia/reperfusion (I/R) injury is marked by oxidative stress, inflammation, and neuronal loss. Natural antioxidants such as fish oil and Nigella sativa oil have shown potential neuroprotective properties. This study evaluated the individual and combined effects of these oils in a mouse model of cerebral ischemia.
Methods: Forty mice were randomly assigned to five groups: sham, I/R, Nigella sativa oil, fish oil, and combined treatment. Cerebral ischemia was induced by 17 min bilateral common carotid artery occlusion followed by reperfusion. Treatments were administered orally. Motor coordination and cognitive function were assessed using behavioral tests, while neuronal damage was evaluated histologically. Oxidative stress markers (MDA, GSH, SOD) and the inflammatory cytokine tumor necrosis factor-alpha (TNF-α) were measured in brain tissue.
Results: All treated groups showed significant improvements in motor and cognitive performance compared with the I/R group. Treatments reduced MDA and TNF-α levels and increased GSH and SOD activity. Histopathological analysis revealed attenuated hippocampal neuronal damage, with the combination therapy showing the greatest protection.
Conclusions: Combined fish oil and Nigella sativa oil therapy provides enhanced neuroprotection against cerebral I/R injury and may represent a supportive strategy for reducing ischemic brain damage.
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Citations
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Ethics Approval
The study protocol was approved by the Institutional Animal Ethics Committee of Raj Kumar Goel Institute of Technology (Pharmacy) with approval no. RKGIT/IAEC/2024/08.CRediT authorship contribution
GM conceptualized the study, supervised the investigation, reviewed, and edited the manuscript. PM conducted the experimental work, data analysis, and drafted the manuscript. SS performed statistical analyses, histological examination, and contributed to the manuscript writing. RT critically reviewed and edited the manuscript. MS arranged all the necessary resources, supervised the investigation, and reviewed the manuscript. All the authors reviewed and approved the final version of the manuscript.
Data Availability Statement
All data generated or analyzed during this study are included in this published article.
How to Cite
1.
Mehta G et al. Neurovascular protective effects of nutritional interventions in ischemic brain injury. Bleeding Thromb Vasc Biol [Internet]. 2026 Apr. 10 [cited 2026 Apr. 16];5(2). Available from: https://www.btvb.org/btvb/article/view/436
Copyright (c) 2026 The Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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