Brief Reports
16 February 2023
Vol. 2 No. 1 (2023)
https://doi.org/10.4081/btvb.2023.51

Interaction between adenosine diphosphate receptors and protein-kinase C isoforms in platelet adhesion under flow condition

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Platelet adhesion; ADP-receptors; PKC isoforms.
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Authors

Boris Shenkman
National Hemophilia Center, Sheba Medical Center, Ramat Gan, Israel.
Ivan Budnik
Department of Pathophysiology, Sechenov First Moscow State Medical University, Moscow, Russian Federation.
Yulia Einav
yulia_e@hit.ac.il
https://orcid.org/0000-0001-8222-7695
Faculty of Engineering, Holon Institute of Technology, Holon, Israel.

Adenosine diphosphate (ADP) receptors and protein-kinase C (PKC) isoforms play different role in platelet activity. In the present study, whole blood platelet adhesion at 200 - 1800 s-1 shear rates was investigated by Impact-R system, measuring percent of surface coverage (SC) by platelets. Gradual heightened shear rate par-alleled increase of platelet adhesion. At relatively low shear (200 and 1000 s-1) blockade of neither P2Y1 receptor nor P2Y12 receptor (by A2P5P and 2MeSAMP, respectively) affected SC. At high shear rate (1800 s-1) reduction of SC was observed by 2MeSAMP. Treatment of blood with PKCδ inhibitor (rottlerin) but not PKCα,β inhibitor (Gö6976) diminished platelet adhe-sion. Among all the agents, only combination of 2MeSAMP and rottlerin used at subthreshold concentrations was able to inhibit platelet adhesion under high shear condition. We suggest that platelet agonist-induced P2Y12 and PKCδ signaling essentially stimulates platelet adhesion under flow condition, the important initiating step of thrombin formation.

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Interaction between adenosine diphosphate receptors and protein-kinase C isoforms in platelet adhesion under flow condition . (2023). Bleeding, Thrombosis and Vascular Biology, 2(1). https://doi.org/10.4081/btvb.2023.51
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